XBLOT P100

X-Blot Hybridization Equipment

The XBLOT P100 (CE marked) is a motorized temperature-controlled roller intended for Western (W), Northern (N) and Southern (S) hybridizations as it is able to evenly distribute reagents of interest onto nitrocellulose or nylon (PVDF) filters containing electrophoresed or fractionated proteins (W), DNA (S) or RNA (N). The filters are enclosed within a perfectly sealed plastic envelope allowing the use of a small amount of reagent (just sufficient to keep the filter wet). The continuous movement of the roller facilitates the binding of the antibody to a protein (in case of Western Blot) or of a labelled probe to DNA or RNA (in case of Southern or Northern Blot).

Advantages in using as protein hybridizer XBLOT P100 over the standard method
(Analogous considerations may be as well made for DNA and RNA hybridization)

• Increase signal sharpness and reproducibility

The even distribution of reagents and the constant temperature during the hybridization process avoid precipitate formation and reagent evaporation, thus enhancing results acquisition.

• Marked reduction in hybridization execution time from overnight to few hours
• Reduction in the amount of reagent used and its further reuse

Example:
6 filters tested per day: total of 120 filters per month
Recovery of the solution containing the primary antibody and re-use for other 2 times
Total costs
Plastic envelope hybridization using XBLOT P100: 100.4€/month
Classic hybridization in tray: 1010€/month
Total money saving of about 900€/month with XBLOT P100 hybridization!

• Adjustable temperature according to the antibody specificity and execution temperature

Other advantages:

• Reduction in time necessary for plate developing.
• Marked recovery of the reagents because of the absence of material evaporation.
• Possibility to handle various filters simultaneously.

Technical specifications:

• Temperature-controlled plate by Peltier module (Operating temperature: 0-50°C).
• 20cm x 29.7cm plate to accommodate several filters simultaneously, also stratified.
• Adjustable roller speed.
• Adjustable roller travel distance according to the area occupied by the plastic envelopes.
• Steel roller mounted on radial ball-bearing and with soft PVC coating highly resistant to wear and chemical agents (Operating temperature: 0-38°C).

Optional accessories:

• Steel roller mounted on radial ball-bearing and with rubber sheath coating for use at higher temperature (Operating temperature: 0-50°C).
• Manual sealer for plastic envelopes with temperature controller (Max. seal: 300mm)