BioRep offers molecular biology services all extensively quality controlled. Starting from DNA and RNA analysis performed by Polymerase Chain Reaction and subsequent sequencing or electrophoretic separation based on nucleic acid fragments size, BioRep Molecular Biology Laboratories have optimized a wide variety of genetic tests, allowing analysis and studies ranging from molecular genetics and molecular oncology (predictive tests, early diagnosis) to pharmacogenetics (individual response and adverse reaction to drugs), to detection of genetic markers for specific diseases (hereditary trombophylia). BioRep staff can offer complete scientific and technical consultancy service for setting up methods for customized nucleic acid analysis, encompassing nucleic acids purification from different sources, primers design, amplification and final sequence analysis.
The DNA sequencing service provides automated processes to obtain high quality sequences.The sequence technology is based on fluorescent dye terminator chemistry and 3500 DNA Analyser (Applied Biosystems).
Different options for DNA sequencing are available:
Diagnostic mutation detection protocols have already been standardized for several genes (KRas, BRaf, EGFR, BRCA); customized protocols for specific mutation detection can also be set up. Special needs and requests can be studied and discussed.
DHPLC is used to scan for new polymorphisms (i.e. SNP, Single Nucleotide Polymorphism) and to determine the allele(s) of a known polymorphism.
Microbiology laboratory can offer micoplasma detection in cell cultures using PCR, Hoechst stain and growth in specific selective media. Through DNA sequencing capability, BioRep can provide a service for genus and species identification of mycoplasmas, bacteria and fungi