The cell biology laboratory offers a wide range of services ranging from tissue-specific cell isolation to reprogramming and differentiation of hiPS. All materials used as well as being of the highest quality are tested before entering the production cycle. The techniques used have been developed, standardized by our staff over the years and are constantly improved and perfected thanks to the continuous updating of our staff through participation in training courses, seminars and internships. In addition to offering technical and scientific advice, the laboratory is able to develop services built according to the specific needs of the customer.
Isolation and lymphoblastoid line creation from blood
Mononuclear blood cells are isolated by concentration gradient and then immortalized using Epstein-Barr virus. The lymphoblastoid line is expanded in the absence of antibiotics until enough cells are obtained for freezing. The cells are frozen in the presence of DMSO in heat-sealed cryovial and through a controlled freezing ramp. Before being distributed, the stock is subjected to a strict quality control which verifies its viability and the absence of bacterial, fungal and mycoplasma contamination. The identity of the line is also verified by genotyping.
In addition, it is possible to perform specific tests for the identification of mycoplasma contamination such as RT-PCR, Hoecst staining and growth on specific soils. In addition to the above mentioned quality controls, it is also possible to create a stock that meets all customer requirements (number of ampoules and cells, type of cryoprotectant, etc.).
BioRep is committed in this case to carry out the distribution and to follow the recipient step by step both as regards the resolution of any bureaucratic and technical problems. Each single clone is also sent together with a detailed data sheet and the technical staff offers continuous support for any need.
Generation of hiPSCs by episomal vectors
BioRep provides the reprogramming service of somatic cells (PBMC or fibroblasts) in iPS. Samples can come from both healthy donors and carriers of specific diseases (e.g. Parkinson’s, Alzheimer’s, ALS, diabetes, etc.). Each cell line created is subjected to different quality controls including sterility, genomic stability, proliferative capacity, pluripotency through in vitro differentiation of embryonic germ leaflets and expression of specific markers through molecular and immunohistochemical analysis.
Differentiation of hiPSCs
According to the different needs of the customer, BioRep is able to offer a differentiation service of hiPS cells into different types of cells. Also in this case the cell line obtained will be subjected to different quality controls such as the expression of specific markers through molecular analysis and immunohistochemistry.